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Perpetua: The Journal of Undergraduate Research at UAH

College

College of Nursing

Abstract

The number of bacterial strains resistant to antibiotics is growing exponentially. Antibiotics are often prescribed more than needed due to the delay period in diagnosing the pathogen and giving treatment. This delay often pushes prescribers to give a broad-spectrum antibiotic or pushes them to make the patient wait for the proper treatment, in turn allowing the bacterium to potentially mutate. This creates a need for a more rapid, easily used, and cost effective method of identifying pathogens. The objective of this study is to validate the CAPTURE™ method and its ability to identify pathogens, reduce the mechanical processing time, and optimize the sample preparation and lysis protocols.

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