Date of Award
2019
Document Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Biotechnology Science and Engineering
Committee Chair
Robert McFeeters
Committee Member
Emanuel Waddell
Committee Member
Roy Magnuson
Committee Member
Luis Cruz-Vera
Committee Member
Eric Mendenhall
Subject(s)
Hydrolases--Analysis., DNA-protein interactions.
Abstract
Putative peptidyl-tRNA hydrolase domain containing 1 (PTRHD1) is a eukaryotic protein predicted to be a Pth2-like peptidyl-tRNA hydrolase. Peptidyl-tRNA hydrolases (Pth) perform the essential function of recycling tRNA from peptidyl-tRNA in the event of translational failure. This function is ubiquitous in all domains of life. Homologues of bacterial (Pth1) and archaeal (Pth2) Pths are present in eukaryotes; however, simultaneous knockout of Pth1 and Pth2 has no effect on cell viability, suggesting the presence of additional proteins in eukaryotes that contain Pth activity. PTRHD1 was proposed as one such eukaryotic Pth and was tested for Pth hydrolase activity. While PTRHD1 did not display hydrolase activity, the protein was found to bind DNA. Therefore, PTRHD1 and DNA interactions were further explored using isothermal titration calorimetry and electrophoretic mobility shift assays, but further optimization and testing is needed to fully explore PTRHD1 interactions with DNA.
Recommended Citation
Burks, Geordan L., "Characterization of putative peptidyl-tRNA hydrolase domain containing 1" (2019). Dissertations. 170.
https://louis.uah.edu/uah-dissertations/170