Date of Award
2013
Document Type
Dissertation
Degree Name
Doctor of Philosophy (PhD)
Department
Biotechnology Science and Engineering
Committee Chair
Roy Magnuson
Committee Member
Debra M. Moriarity
Committee Member
Joseph Ng
Committee Member
Marshall Schreeder
Committee Member
Jian Han
Subject(s)
Polymerase chain reaction, RNA microarrays, Medical screening, Cancer--Diagnosis, RNA--Analysis, Gene expression
Abstract
Ligation-dependent PCR, when coupled with a bead-based detection array, comprises a method of nucleic acid detection that utilizes enzymes and equipment already being employed successfully in clinical labs. The process can be multiplexed in order to generate a profile of the sequences of interest. When applied to microRNA profiling, the ligation step requires modification to improve the efficiency of the T4 DNA ligase employed. With optimization, the assay has the potential to distinguish and detect multiple microRNA species simultaneously, providing a clinically adaptable method for microRNA profiling. The flexibility of the assay format would allow modification for a nearly limitless repertoire of disease-specific panels.
Recommended Citation
Johnston, Bobbi Ellen, "Ligation-dependent multiplex PCR with bead-based array detection comprises a clinically adaptable method of microRNA profiling" (2013). Dissertations. 6.
https://louis.uah.edu/uah-dissertations/6