Ligation-dependent multiplex PCR with bead-based array detection comprises a clinically adaptable method of microRNA profiling
Date of Award
Doctor of Philosophy (PhD)
Biotechnology Science and Engineering
Debra M. Moriarity
Polymerase chain reaction., RNA microarrays., Medical screening., Cancer--Diagnosis., RNA--Analysis., Gene expression.
Ligation-dependent PCR, when coupled with a bead-based detection array, comprises a method of nucleic acid detection that utilizes enzymes and equipment already being employed successfully in clinical labs. The process can be multiplexed in order to generate a profile of the sequences of interest. When applied to microRNA profiling, the ligation step requires modification to improve the efficiency of the T4 DNA ligase employed. With optimization, the assay has the potential to distinguish and detect multiple microRNA species simultaneously, providing a clinically adaptable method for microRNA profiling. The flexibility of the assay format would allow modification for a nearly limitless repertoire of disease-specific panels.
Johnston, Bobbi Ellen, "Ligation-dependent multiplex PCR with bead-based array detection comprises a clinically adaptable method of microRNA profiling" (2013). Dissertations. 6.