Date of Award
2021
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
Committee Chair
Ahmed Lawan
Committee Member
Jerome Baudry
Committee Member
Bruce Stallsmith
Subject(s)
Phosphatases, Glucose--Metabolism, Lipids--Metabolism, Metabolism--Disorders--Animal models
Abstract
The function of MKP-2 in metabolism and metabolic diseases remains largely unknown. In obese conditions mitogen-activated protein kinase (MAPK) pathway get dysfunctional and MAP kinase phosphatases (MKPs) play major roles in regulating the activities of MAPKs. We examined the regulation of MKP-2 expression in vivo, under physiological and pathological conditions, in the liver and other insulin responsive tissues using diet-induced obesity models using novel MKP-2 knockout mice. Also, we examined MKP-2 expression in vitro using insulin sensitive cell lines. We found that MKP-2 is expressed in insulin-sensitive tissues/cells and overexpression may delay muscle cell differentiation. Our novel data demonstrate that under high fat-diet feeding conditions MKP-2 knockout mice are insulin sensitive and resistant to diet-induced obesity compared to MKP-2 wild type mice. We demonstrate that, in obese conditions, MKP-2 is overexpressed in the liver resulting in inactivation of MAPKs and thereby promoting development of obesity, fatty liver and metabolic dysfunction.
Recommended Citation
Fernando, Yanthrawaduge Savanie Sulakshana Devsirini, "Role of MKP-2 in glucose metabolism and stress-induced insulin resistance" (2021). Theses. 362.
https://louis.uah.edu/uah-theses/362