Date of Award

2013

Document Type

Thesis

Degree Name

Master of Science in Engineering (MSE)

Department

Chemical and Materials Engineering

Committee Chair

Emanuel A. Waddell

Committee Member

Krishnan Chittur

Committee Member

C. P. Chen

Subject(s)

Microfluidics, Miniature electronic equipment, Microfluidic devices, Microelectronics, Micromechanics, Biochips, Biotechnology--Materials

Abstract

The purpose of the research was to construct a working lab-on-a-chip device constructed from polydimethylsiloxane (PDMS) in a Y-channel design that consisted of two generated magnetic fields: one localized below the channel, and an external field outside of the device generated through two rare earth magnets. The external field provided a method to attract the superparamagnetic beads while the localize field influenced attractive and replusive forces in the channel. The localized magnetic field allowed for controlling the direction the superparamagnetic beads in the channel to a predetermined well. The localized magnetic field generated a field of 5.7 x 10-3 mT. The fluid flow within the device was electroosmotically driven. The electroosmotic flow in the channel was 3.13x10-7 cm2/V s. The superparamagnetic bead traveled down the channel at 8.98 x 10-5 m/s. Beads were identified with Raman spectroscopy.

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