Date of Award
2023
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biological Sciences
Committee Chair
Ahmed Lawan
Committee Member
Lawana Adcock
Committee Member
Jerome Baudry
Subject(s)
Phosphatases, Blood platelets--Activation--Research, Obesity, Nutritionally induced diseases
Abstract
Mitogen-activated protein kinases (MAPKs) regulate metabolic homeostasis and MAPK dysfunction promotes metabolic diseases. MAPK phosphatases (MKPs) regulate MAPKs and are implicated in obesity and platelet activation. To elucidate the role of MKP-2 in platelet function and NAFLD, tail bleed, platelet isolation, and western blot assays were performed. MKP-2 KO mice and control mice were fed either a chow or 12- 13 week high fat diet (HFD), a 10 week iron deficient (ID), or an iron supplement (IS) regimen. On the chow diet, the bleeding time was significantly reduced in female MKP-2 KO mice. Male MKP-2 KO IS mice showed significantly increased bleed time. HFD caused male MKP-2 KO mice to weigh significantly less and have significantly lower hemoglobin levels. Male MKP-2 KO platelets also showed increased activation of p38 MAPK and ERK and expression of SDF-1. Overall, these results demonstrate a relationship between the MKP-2 and platelet activity.
Recommended Citation
Secunda, Cassandra, "The impact of MKP-2 deficiency on hematological parameters in diet challenged mice" (2023). Theses. 613.
https://louis.uah.edu/uah-theses/613