Date of Award

2024

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Committee Chair

Zach Culumber

Committee Member

Matt Niemiller

Committee Member

Bruce Stallsmith

Research Advisor

Zach Culumber

Subject(s)

Eastern mosquitofish--Variation, Eastern mosquitofish--Color, Melanins, Variation (Biology)

Abstract

Genetic variation often precedes adaptation, so the mechanisms that maintain this variation are a central topic in evolutionary biology. Gambusia holbrooki, or eastern mosquitofish, possesses a rare polymorphism for melanistic coloration. Melanics and typical silver males were examined for differences in behavioral and physiological traits that may impact the persistence of the melanism trait. No differences in standard metabolic rate were found via closed-chamber respirometry, but a detour task indicated that melanic males possess greater cognitive flexibility. Effects of the social environment on stress and reproductive traits were also evaluated by pairing males and comparing cortisol levels and sperm quality to individual baselines. These controlled social pairings revealed no significant relationships between social factors, stress, and reproductive traits. Future work may better illustrate the role of environmental factors on the persistence of melanism by thoroughly characterizing both competitive and reproductive interactions and evaluating potential physiological mechanisms underlying differences in cognition.

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