Date of Award

2025

Document Type

Thesis

Degree Name

Master of Science (MS)

Department

Biological Sciences

Committee Chair

Matthew L. Niemiller

Committee Member

Zachary Culumber

Committee Member

Annette Summers Engel

Research Advisor

Matthew L. Niemiller

Subject(s)

Groundwater animals--Cumberland Mountains, Cave animals--Cumberland Mountains, Caves--Cumberland Mountains, Microbiomes

Abstract

Caves and other subterranean ecosystems are among the most understudied ecosystems globally and house unique and ecologically important biodiversity. While several studies have characterized the microbial communities of respective cave systems, research on microbiomes of cave fauna has been limited. This study investigated microbiomes for aquatic cave fauna in the southern Cumberland Plateau of Tennessee and Alabama, USA. Exoskeleton and skin swabs were taken from three species of cave-dwelling crayfishes, two species of salamanders, and one cavefish. Bacterial operational taxonomic units (OTUs) identified in microbiomes of hosts included primarily members of the phylum Pseudomonadota, but also the phyla Acidobacteriota, Actinomycetota, Bacteroidota, Chloroflexota, Cyanobacteriota, and Planctomycetota. Environmental and land cover/land use (LULC) variables affected microbiome composition and diversity for hosts and the surrounding aquatic environment. Microbiome diversity and composition varied between sites primarily for Southern Cave Crayfish, and to some degree for Southern Cavefish and Tennessee Cave Salamanders.

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